Hi, this is DNA2.0. I will walk you through the click-drag interface of Gene Designer Let’s start by opening up a file On your left-hand side, you’ll find the Library Explorer. Here you can create new elements, It shows a box of existing elements, a box of vectors and a box of codon tables. On your right hand side you’ll find a workspace. In this example we have 2 constructs, one is the T7-SNAP vector, one is the trmA gene. The T7-SNAP vector is built from a good ten or so different elements whereas the trmA gene is just a single element. As you can see, the annotation for each element is shown in the lower left corner. You can add your own elements. View>>Select element Edit and delete elements as you please. So, let’s insert an element. Let’s put it next to the 3′ stuffer. Add New Element. Let’s make that a DNA element. Give it a name, Give it a color, and a sequence, and some annotation. The new element is now found here, you can drag it too, if you prefer to put it in some other location, just by click and drag. Or, change the orientation or what have you You can also just put it into your ‘Elements Not in a Construct’. And delete this from the overall sequence. You can also take an existing element from Say, the His tag. Put it next to the pet gene, the SNAP gene. Or, drag it around as you please. You can change the proportion view, so you see that relative size or just as non-proportion view so you can find all the sequences easily. Drag it to whatever location. You can also from this pull-down menu, start new projects, open existing projects ‘Save Projects As’ and so on Here’s the import and export function. In the Edit View you can undo, you can Cut, Copy, Paste. Tools pulldown menu, there’s a back translate tool, there’s a cloning tool, there’s a DNA sequencing oligo tool There’s a report function pull-down. you can also use this pull-down to configure your restriction sites. back translation profile, motifs, and so on. So here you see the icon view for the construct, in a proportional view. You can also look at the sequence view for these constructs. So here you have the sequence view for the T7-SNAP. This tab is for the trmA gene and within the T7-SNAP because it is a circular plasmid, you can use the telescope view to orient yourself. You can look on the first level screen through the plasmid Second level drill down in a certain region and the corresponding DNA sequence and amino acid sequence, so you can change the codons as you please, by just clicking on them You can find sequences using the Find function, either search by motifs, by restriction sites, or find open reading frames. So, let’s go find a couple of restriction sites. Click Find. Here’s a list of restriction sites not found in the sequence. and these ones are found, so let’s go and locate this guy, here’s a BsaI site Here’s the restriction recognition site Here’s the cut site. You can navigate through sequence the same way. Here’s the EagI cut and recognition.